Group "mRNA metabolism"

The "mRNA metabolism" research group headed by Prof. Dr. Andreas Kulozik focuses on basic and translational aspects of RNA biology in common diseases such as the hemoglobin disorders, thrombosis, inflammation and childhood cancer. Research is conducted within the MMPU (Molecular Medicine Partnership Unit) in close cooperation with the research group headed by Prof. Dr. Matthias Hentze at the European Molecular Biology Laboratory (EMBL).

Specific areas of interest include the mechanism of nonsense-mediated mRNA decay (NMD, degradation of mRNAs with a premature stop codon), one of the cell’s key mRNA quality control pathways to limit the synthesis of faulty proteins, as well as the regulation of mRNA 3’ end processing and its response to stress.

We are also using cell biological, molecular biological, biochemical as well as transcriptomic and proteomic approaches to investigate the role of RNA-binding proteins (RBP) in cancer and their responses to pharmacological intervention.


Mitarbeiter

  • Prof. Dr. Andreas Kulozik (Group leader)
  • PD Dr. rer. nat. Gabriele Neu-Yilik (Staff scientist)
  • Dr. Michael Backlund (Postdoc)
  • Dr. Daria Lavysh (Postdoc)
  • Dr. rer.nat. Yang Zhou (Postdoc)
  • Jonas Becker (PhD student)
  • Annika Brosig (PhD student)
  • Beate Amthor (Technician)
  • Nicole Dickemann (Technician)
  • Margit Happich (Technician)
  • Gabriele Tolle (Technician)

Prof. Dr. med. Andreas Kulozik, PhD

Director "Clinical Pediatric Oncology"

Postal address:
Clinic for Pediatric Oncology, Hematology, and Immunology
Center for Child and Adolescent Medicine
Jutta Mattern
Im Neuenheimer Feld 430
D-69120 Heidelberg
Germany

Selected publications

1. Gabriele Neu-Yilik, Etienne Raimondeau, Boris Eliseev, Lahari Yeramala, Beate Amthor, Aurélien Deniaud, Karine Huard, Kathrin Kerschgens, Matthias W. Hentze, Christiane Schaffitzel, Andreas E. Kulozik EMBO J. 2017 Sep 12. Dual function of UPF3B in early and late translation termination,  doi: 10.15252/embj.201797079, PMID:28899899

2. Haberman N, Huppertz I, Attig J, König J, Wang Z, Hauer C, Hentze MW, Kulozik AE, Le Hir H, Curk T, Sibley CR, Zarnack K, Ule J. Genome Biol. 2017 Jan 16.  Insights into the design and interpretation of iCLIP experiments. doi: 10.1186/s13059-016-1130-x. PMID: 28093074

3. Hauer C, Sieber J, Schwarzl T, Hollerer I, Curk T, Alleaume AM, Hentze MW, Kulozik AE. Cell Rep. 2016 Aug 9.  Exon Junction Complexes Show a Distributional Bias toward Alternatively Spliced mRNAs and against mRNAs Coding for Ribosomal Proteins. doi: 10.1016/j.celrep.2016.06.096. Epub 2016 Jul 28. PMID: 27475226

4. Hollerer I, Curk T, Haase B, Benes V, Hauer C, Neu-Yilik G, Bhuvanagiri M, Hentze MW, Kulozik AE. RNA. 2016 Jul 12. The differential expression of alternatively polyadenylated transcripts is a common stress-induced response mechanism that modulates mammalian mRNA expression in a quantitative and qualitative fashion. PMID: 27407180

5. Sieber, J., Hauer, C., Bhuvanagiri, M., Leicht, S., Krijgsveld, J., Neu-Yilik, G., Hentze, M.W., Kulozik, A.E. Molecular & Cellular Proteomics 2016 Feb 20  Proteomic analysis reveals branch-specific regulation of the unfolded protein response by nonsense-mediated mRNA decay pii: mcp.M115.054056.

6. Danckwardt S, Gantzert AS, Macher-Goeppinger S, Probst HC, Gentzel M, Wilm M, Gröne HJ, Schirmacher P, Hentze MW, Kulozik AE. Mol Cell. 2011 Feb 4 p38 MAPK controls prothrombin expression by regulated RNA 3' end processing. doi: 10.1016/j.molcel.2010.12.032. PMID: 21292162

7. Danckwardt S, Hentze MW, Kulozik AE.
J Mol Med (Berl). 2013 Aug 17. Pathologies at the nexus of blood coagulation and inflammation: thrombin in hemostasis, cancer, and beyond. doi: 10.1007/s00109-013-1074-5. PMID: 23955016

8. Bhuvanagiri M, Lewis J, Putzker K, Becker JP, Leicht S, Krijgsveld J, Batra R, Turnwald B, Jovanovic B, Hauer C, Sieber J, Hentze MW, Kulozik AE.
EMBO Mol Med. 2014 Oct 15. 5-azacytidine inhibits nonsense-mediated decay in a MYC-dependent fashion.
pii: e201404461. doi: 10.15252/emmm.201404461.